Globitermes brachycerastes Han, 1987 短角球白蚁

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1. Suicidal defensive behaviour by frontal gland dehiscence in Globitermes sulphureus Haviland soldiers (Isoptera)

   Christian Bordereau, Alain Robert, V. Van Tuyen, Alexis Peppuy · 1997 · Insectes Sociaux

   摘要

2. Screening of multimeric β-xylosidases from the gut microbiome of a higher termite, Globitermes brachycerastes

   Chunyan Liu, Gen Zou, Xing Yan, Xuguo Joe Zhou · 2018 · International Journal of Biological Sciences

   摘要

   Termite gut microbiome is a rich reservoir for glycoside hydrolases, a suite of enzymes critical for the degradation of lignocellulosic biomass.To search for hemicellulases, we screened 12,000 clones from a fosmid gut library of a higher termite, Globitermes brachycerastes.As a common Southeastern Asian genus, Globitermes distributes predominantly in tropical rain forests and relies on the lignocellulases from themselves and bacterial symbionts to digest wood.In total, 22 positive clones with β-xylosidase activity were isolated, in which 11 representing different restriction fragment length polymorphism (RFLP) patterns were pooled and subjected to 454 pyrosequencing.As a result, eight putative β-xylosidases were cloned and heterologously expressed in Escherichia coli BL21 competent cells.After purification using Ni-NTA affinity chromatography, recombinant G. brachycerastes symbiotic β-xylosidases were characterized enzymatically, including their pH and temperature optimum.In addition to β-xylosidase activity, four of them also exhibited either β-glucosidase or α-arabinosidases activities, suggesting the existence of bifunctional hemicellulases in the gut microbiome of G. brachycerastes.In comparison to multimeric protein engineering, the involvement of naturally occurring multifunctional biocatalysts streamlines the genetic modification procedures and simplifies the overall production processes.Alternatively, these multimeric enzymes could serve as the substitutes for β-glucosidase, β-xylosidase and α-arabinosidase to facilitate a wide range of industrial applications, including food processing, animal feed, environment and waste management, and biomass conversion.

3. MORTALITY OF GLOBITERMES SULPHUREUS (FAMILY: TERMITIDAE) AGAINST GLIRICIDIA SEPIUM (FAMILY: FABACEAE)

   Fauziah Abdullah, Mohd Shukri Mohd Sabri, Davindran Rajandram · 2015 · Serangga

   摘要

   The mortality of Globitermes sulphureus against essential oil Gliricidia sepium was investigated. High mortality was shown by hexane extract compared to methanolic extract and DCM extract. 50% mortality was achieved after 15 hours exposure towards 2.96 μg/ml (LD) of G. sepium. neophytadine and coumarine are the compounds that cause mortality to termites.

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